Application

Western blot: 1-10 µg/mL using ECL.
Immunohistochemistry: Not tested. It is recommended that the antibody be tried at 2-20 µg/mL
ELISA: 1:10,000-1:100,000 using 50-100 ng of control peptide/well.
Optimal working dilutions must be determined by end user.

Research CategoryNeuroscience

Detect Neuronal pentraxin 2 using this Anti-Neuronal pentraxin 2 Antibody validated for use in ELISA & WB.

Research Sub CategorySynapse & Synaptic Biology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Neuronal Pentraxins: A new family of putative integral membrane pentraxins or Neuronal pentraxins identified through interaction with a presynaptic snake venom toxin taipoxin. NPX1, NPX2 and NPXR (Neuronal Pentraxin Receptor), the three neuronal pentraxins represent a novel neuronal uptake pathway that may function during synapse formation and remodeling. The N-terminal half of neuronal pentraxins are 20-30% identical to previously identified pentraxins (CRP and SAP), the three neuronal pentraxins are 50% identical to each other and are significantly larger than the classical pentraxins (>50 versus 30kDa), suggesting that they may have additional novel functions. NPX2 (Neuronal Pentraxin 2), 429aa in mouse, 432 in rat and 431 in human (chr 7q21), mainly expressed in brain, pancrease, liver and testis. It is likely to play role in the modification of cellular properties that underlie long-term plasticity. NPX1, NPX2 has potential N-linked glycosylation sites.

Immunogen

Synthetic peptide near the N-terminus of mouse NPX2

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Liquid in 100 mM Tris, pH 7.5 and 0.1% BSA.

ImmunoAffinity Purified

Format: Purified

Specificity

Other species have not yet been tested. The immunogen sequence is 100% conserved in rat, and 94% in human NPX2 and 82% in guinea pig apexin precursor P50.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.